Core Molecular Techniques and Technologies (HLS310)

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Core Molecular Techniques and Technologies (HLS310)

Module Objective

By the end of the training period trainees will, in respect to core molecular techniques and technologies relevant to Molecular Pathology of Acquired Disease be able to:

  • analyse, synthesise, evaluate and apply knowledge
  • perform a range of technical and clinical skills and procedures and
  • demonstrate the attitudes and behaviours necessary for professional practise as a Consultant Clinical Scientist dealing with the complexities, uncertainties and tensions of professional practise at this level.

By the end of the training period the trainee will be able to demonstrate the ability to analyse, evaluate and synthesise relevant knowledge and its application to their professional practice in relation to:

  • basic principles of Molecular Biology
  • human genetic variation
  • genetic basis, clonal evolution and molecular heterogeneity of cancer
  • germline vs somatic genetics
  • DNA, RNA and protein extraction (microbes, blood, fresh and formalin-fixed tissue samples)
  • Polymerase Chain Reaction (PCR), Reverse-Transcriptase PCR (RT-PCR) and quantitative PCR (qPCR)
  • primer design
  • nucleotide and protein gel electrophoresis
  • restriction endonucleases
  • DNA hybridisation techniques: Southern Blotting, Fluorescence-in-situ Hybridisation (FISH) and Array Comparative Genomic Hybridisation (aCGH)
  • gene expression profiling
  • Capillary (Sanger) sequencing of DNA: principles and applications
  • Next Generation Sequencing (NGS) technologies: principles and applications (including NGS library preparation, nucleotide barcoding, genome sequencing, target enrichment/exome sequencing and paired- end sequencing)
  • RNA sequencing 
  • analysis and interpretation of NGS data
  • assays for the detection of minimal residual disease in haematological and solid tumours
  • screening assays for neoplasia and pre-neoplasia.