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Learning outcome |
Title |
Knowledge |
1 |
1,2 |
Identify the appropriate tests for at least two H&I clinical presentations. |
- The repertoire, specimen requirements, referral pattern and storage, ordering, reference ranges and turnaround times of the methods used in H&I.
- The major clinical users of the H&I service.
- The range of investigative techniques used in H&I and their application.
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2 |
1,2 |
Receive, label and store a range of immunological specimens
- Serum specimens
- DNA specimens
- Lymphocyte specimens
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- Minimum data set required for identification of samples and the importance of ensuring that this is complete and correct.
- Factors affecting sample integrity and appropriate corrective action.
- Procedures for handling samples which may contain category 2,3, and 4 pathogens.
- Types and implications of hazards and risks associated with handling of specimens and relevant control measures.
- The quality management process that ensures the correct location and storage of documentation and specimens at each stage of process.
- Safe laboratory practices including principles of decontamination of equipment and work areas.
- Local and national Health and Safety policies and procedures and their application.
- Relevant records, their importance and how to complete these correctly.
- Correct use of manual and computerised systems for generating labels for the products and component.
- Specimen preservation, distribution, separation, storage and disposal procedures.
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3 |
3,5 |
Select and apply appropriate control materials. |
- Selection and use of suitable and appropriate controls.
- Use and application of reagents for analysis.
- Correct conditions and locations for storage of test reagents.
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4 |
3,5 |
Use automated methods, techniques and instrumentation to include at least four of the following:
- Immunoassay
- Electrophoresis
- Immunoflourescence
- Microscopy
- Flow cytometry
- X map technology
- Complement dependent cytotoxicity
- PCR
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- Capabilities and limitations of methods, techniques and equipment.
- Use, care, monitoring, calibration and routine maintenance of clinical immunology laboratory equipment to include (relevant to automated methods available) from the following list:
- Pipettes
- Balances
- Centrifuges
- Refrigerators
- Water baths
- Incubators
- pH meters
- freezers
- automated analysers
- flow cytometer
- thermal cycler
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5 |
1,4,5 |
Interpret laboratory data in the light of clinical details on patients with common disorders where transplantation is the therapy of choice, including at least 2 of the following;
- Autoimmune disorders
- Immunodeficiency disorders
- Renal disorders
- Other solid organ disorders
- Haematological malignancies
- Disease associations
- Drug hypersensitivity
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- Organisation and components of the immune system.
- Ranges and values needed for interpretation of results.
- Cellular components (lymphocytes; granulocytes; monocytes/macrophages).
- Humoral components (autoantibodies, the range of autoantibodies and the role they play in autoimmune disease; immunoglobulins and the importance of their levels and their absence; complement and the importance of their levels and their absence).
- Central molecules of the immune system (major histocompatibility molecules, classes I ⅈ Cluster of Differentiation (CD) molecules/cell surface markers; receptor molecules; recognition molecules; adhesion molecules; effector molecules).
- Antigen presentation.
- Innate immune response (endothelial cells; neutrophils; macrophages; natural killer cells; complement).
- Adaptive immune response (antigen processing; dendritic cells; T cell responses; B cell responses; primary and secondary responses; vaccination/immunisation).
- The adaptive immune system routine assays which can be usefully examined in an H&I laboratory.
- Outcome of immune responses (immunity/immunological memory; direct and indirect functions of antibodies).
- Major assays performed in an H&I laboratory.
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6 |
1,2,4 |
Produce interpretative reports on laboratory investigations. |
- The information to be included in an interpretative report.
- How to construct an interpretative report and the format required for presentation.
- Limits of responsibility in the authorisation and issue of interpretative reports.
- Clinical conditions which may require urgent action and how to instigate such action.
- Normal and abnormal results and their significance to clinical question or condition.
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7 |
1,3 |
Control infection risks in accordance with departmental protocols. |
- Protocol for hand washing and awareness of how effective hand washing contributes to control of infection.
- Protocols and requirements for hygiene and infection control related to the relevant range of investigations, including preparation, conduct and completion of investigation.
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8 |
1,3 |
Minimise risks and hazards in compliance with Health and Safety policies. |
- The relevant health and safety regulations for laboratory and clinical investigations.
- Any specific health and safety regulations required in H and I, taking into account the sample and the investigation.”
- The potential hazards and risks and the actions to be taken to minimise these.
- Responsibilities and scope of practice of laboratory personnel involved in performing investigations and reporting those investigations to users.
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