Module - Immunogenetics (SLS139)

STP

Aim of this module

This module will provide the trainee with knowledge and understanding of the pathophysiology, clinical presentation and management of patients with haematological malignancies, transplantation and appropriate investigations. In the work-based module they will be expected to apply this knowledge as they learn to perform relevant laboratory methods and gain experience of the interpretation of patient results in a variety of clinical settings.

This module will provide the trainee with the practical application of knowledge and skills relating to the scientific principles of molecular techniques available for HLA typing and their applications for different clinical conditions.

Work-based learning outcomes


  1. Select and perform HLA typing methods and HLA loci to be tested for solid organ and haematopoietic stem cell transplantation, associated diseases and pharmacogenetic reactions according to current practice.
  2. Prepare good quality DNA template for HLA typing techniques.
  3. Identify the correct specimen tubes for HLA typing and be able to troubleshoot issues around DNA extraction.
  4. Perform all aspects of the current range of molecular techniques available for immunogenetic testing.
  5. Report on the results from a range of current techniques available for immunogenetic testing.
  6. Apply the specific accreditation standards governing the use of molecular techniques.
  7. Select and perform the appropriate HLA typing resolution in accordance with relevant standards required for different clinical applications.
  8. Work in partnership with other clinical specialisms in the investigation disease association and pharmacogenetics and patients referred for transplantation and, where appropriate with service users and support staff.

Work-based Competencies


Learning outcome Title Knowledge
1 1,3

Receive samples for HLA typing and enter onto laboratory IMS.

  • Minimum data set required for identification of laboratory samples.
  • Factors affecting sample integrity and appropriate corrective action.
  • Correct use of manual and computerised systems for entering laboratory samples.
2 1,2,3

Extract and quantify DNA from samples according to local protocol.

  • Local protocols.
  • Principles of DNA extraction, quantification and purity assessment.
  • Impact of sample source.
  • Safe handling of biological materials.
3 1,2,3,4

Use local molecular based techniques in accordance with quality standards for intermediate resolution HLA typing.

  • How to assure the quality of personal practice.
  • Clinical indications for which HLA typing is required.
  • The local repertoire, source of requests and turnaround times for investigations performed by the training H&I laboratory.
  • Internal quality controls and external quality assessment measures appropriate to the methods used.
  • Correct and safe use of equipment used.
  • Principles of PCR.
  • Principles of HLA typing assays, for example:
    • Sequence specific primers (SSP)
    • DNA probe based hybridisation
    • Real time PCR
    • Sequence based typing (SBT)
    • Next generation sequencing
4 1,2,3,4,6,7

Use local molecular based techniques in accordance with quality standards for High resolution typing.

  • How to assure the quality of personal practice.
  • Clinical indications for which HLA typing is required.
  • The local repertoire, source of requests and turnaround times for investigations performed by the training H&I laboratory.
  • Internal quality controls and external quality assessment measures appropriate to the methods used.
  • Correct and safe use of equipment used.
  • Principles of PCR.
  • Principles of HLA typing assays, for example:
    • Sequence specific primers (SSP)
    • DNA probe based hybridisation
    • Real time PCR
    • Sequence based typing (SBT)
    • Next generation sequencing
5 1,2,5,6,7

Interpret data from HLA typing tests using current nomenclature and produce an HLA typing report for clinical colleagues.

  • How to assure the quality of personal practice.
  • Current HLA nomenclature.
  • Use of National and International standards for reporting of HLA types.
  • Correct use of interpretation software.
  • Correct use of manual and computerised systems for recording test results.
  • Correct use of manual and computerised systems for generating laboratory reports.
  • Factors influencing interpretation of HLA typing data.
  • The need to manage records and all other information in accordance with applicable legislation, protocols and guidelines.
6 1,5,6,7

Produce an interpretive genotyping report for a patient referred for disease association and pharmacogenetic reactions.

  • How to assure the quality of personal practice.
  • The proposed mechanisms for disease association and pharmacogenetic reactions.
  • The clinical impact of positive/negative or presence/ absence of the associated HLA for the patient.
  • The need to manage records and all other information in accordance with applicable legislation, protocols and guidelines.
7 8

Work in partnership with other clinical specialisms in the investigation of disease association and pharmacogenetics and patients referred for transplantation and, where appropriate with service users and support staff.

  • The need to build and sustain professional relationships as both an independent practitioner and collaboratively as a member of a team.
  • Methods of effective communication.
8 8

Communicate effectively with the healthcare team recognising and responding appropriately to situations where it is necessary to share information to safeguard service users or the wider public.

  • How communication skills affect assessment of, and engagement with, service users and how the means of communication should be modified to address and take account of factors such as age, capacity, learning ability and physical ability.
  • Situations where it is necessary to share information to safeguard service users or the wider public.

Work-based assessment


Complete 2 Case-Based Discussion(s)
Complete 2 of the following DOPS and/or OCEs
Type Title
DOPS Prepare a blood sample for molecular HLA testing
DOPS Quantify and assess DNA samples for purity
DOPS Perform HLA typing using local molecular methods
DOPS Prepare an interpretive HLA report
DOPS Perform tests for a patient referred for disease association or pharmacogenetic reactions
OCE Attend a multidisciplinary review meeting at which laboratory results are presented and describe to colleagues what they mean.
OCE Take a patient history