Clinical Biochemistry Investigation of Major Organ Function (SLS100)

10 credits

Aim of this module

This module will provide the trainee with the knowledge and understanding of the normal physiology of the major organs and the biochemical parameters in common use for the investigation and management of major organ dysfunction. In the work-based module they will be expected to apply this knowledge as they learn to perform common methods used in the investigation of major organ function and gain experience of the interpretation of patient results in a variety of clinical settings.

  1. Interpret routine requests for clinical biochemistry investigations of major organ function in the correct clinical context and process the specimens that accompany those requests.
  2. Perform a range of laboratory and point-of-care techniques (POCTs) used in the workplace to investigate major organ function.
  3. Apply the principles of internal quality control and external quality assessment and draw conclusions about assay performance.
  4. Report the results of commonly performed clinical biochemistry investigations of major organ function.
  5. Use laboratory IT systems for handling, processing and storage of patient data.
Number Work-based learning outcome Title Knowledge
1 1

Identify the most appropriate test for at least two example clinical presentations, e.g. diabetes, acute coronary syndrome, acute kidney injury etc.

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2 1,5

Use computer software associated with the LIMS and laboratory equipment.

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3 1,5

Receive, label and store routine clinical biochemistry samples.

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4 2,3

Use automated instrumentation (modular systems, elementary robotics and automated immunoassay analysers) in your training laboratory, which incorporates the following techniques:

  • ion selective electrodes
  • spectrophotometry
  • immunoassay
  • enzymology
  • plus one other from:
    • fluorimetry
    • nephelometry/turbidimetry.
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5 2,3

Use manual and semi-automated techniques to specified standard operating procedures, to include:

  • spectrophotometry
  • osmometers
  • urine analysis (e.g. dipsticks or pregnancy tests)
  • HbA1c analysis.
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6 2,3

Use one of the following POCT methods/devices to specified quality standards:

  • blood gas analysers
  • co-oximetry
  • blood glucose meters.
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7 2,3

Perform analysis of the following analytes:

  • albumin
  • creatinine
  • calcium
  • bilirubin
  • transaminases
  • troponin
  • glucose
  • sodium and potassium
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8 4,5

Produce reports using validated results on common clinical biochemistry investigations.

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9 2,3

Control infection risks in accordance with departmental protocols.

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10 2,3

Minimise risks and hazards in compliance with health and safety policies.

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You must complete
1 Case-based discussion(s)
1 of the following DOPS / OCEs
Assessment Title Type
Receive book process and store common clinical biochemistry specimens DOPS
Perform Serum Osmolality DOPS
Undertake IQC procedure acceptance criteria DOPS
Set up a daily analyser and undertake reagent top up DOPS
Perform daily maintenance procedures DOPS
An appropriate activity agreed with your Training Officer OCE

Important information

The academic parts of this module will be detailed and communicated to you by your university. Please contact them if you have questions regarding this module and its assessments. The module titles in your MSc may not be exactly identical to the work-based modules shown in the e-portfolio. Your modules will be aligned, however, to ensure that your academic and work-based learning are complimentary.

Learning Outcomes

  1. Explain normal physiological homeostasis of the major organs.
  2. Describe and explain the pathophysiology and cause of common disorders of the major organs.
  3. Explain the presentation, diagnosis and management of common biochemical disorders of major organ function.
  4. Describe and evaluate the principles of common biochemical measurement techniques used to investigate major organ function.
  5. Describe the design, operation and performance of automated analytical platforms used to investigate major organ function.
  6. Describe the design, operation and performance of point-of-care testing devices supported by the clinical biochemistry laboratory.
  7. Discuss and justify the biochemical investigation of major organ disease in the patient pathway, the correct sampling technique and the use and validity of reference ranges.
  8. Explain and justify the principles of internal quality control (IQC) and external quality assessment (EQA).
  9. Explain the use of laboratory information technology (IT) systems for handling, processing and storage of patient data.
  10. Discuss the partnership of clinical biochemistry to other clinical specialisms in the investigation of disorders of major organs and patient care.

Indicative Content

  • The normal physiology and function of the following major organs: kidney, liver, heart, lungs, bone and pancreas. To include water homeostasis and acid–base balance
  • The clinical and scientific basis of common biochemical markers of function of the kidney, liver, heart, lungs, bone and pancreas
  • The application of common biochemical markers of major organ function to a range of frequently encountered clinical disorders
  • Presentation, diagnosis and management of common clinical biochemical disorders of major organ function
  • The biological and statistical basis of biological variation, reference values and action limits
  • Principles and practice of IQC and EQA
  • Scientific basis of the following techniques: spectrophotometry, osmometry, ion selective electrodes, enzymology, immunochemical techniques, electrophoresis, chromatography and solid phase chemistry
  • Design, operation and performance of automated analytical platforms, including random access, modular, robotics, etc.
  • Design, operation and performance of point-of-care testing devices supported by the clinical biochemistry laboratory