Number |
Work-based learning outcome |
Title |
Knowledge |
1 |
1,5 |
Receive, label and store of a wide range of histopathology specimens. |
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Receive, label and store of a wide range of histopathology specimens.
Knowledge and skills you might demonstrate
- Minimum data set required for identification of samples and the importance of ensuring that this is complete and correct.
- Factors affecting sample integrity and appropriate corrective action.
- Procedures for handling samples which may contain category 2, 3 and 4 pathogens.
- Types and implications of hazards and risks associated with handling of specimens and relevant control measures.
- The quality management process that ensures the correct location and storage of documentation and specimens at each stage of the process.
- Legal and ethical considerations and requirements in respect of examination of tissue, selection of control material and disposal of specimens.
- Infection risk from blood samples and how to deal with fresh tissue samples.
- Safe laboratory practices, including principles of decontamination of equipment and work areas.
- Specimen acquisition, viability, collection and delivery, including renal biopsies and bullous skin diseases.
- Quality assurance procedures and their application.
- Local and national health and safety policies and procedures and their application.
- The range of imaging procedures available and their use, including storage of images.
- Correct and safe use of imaging equipment and processing of X- rays or photographic films if required.
- The dissection of FRCPath category A, B and C specimens.
- Principles and practice of fixation.
|
2 |
1 |
Prepare and use a microtome on a range of tissue samples within different embedding materials. |
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Prepare and use a microtome on a range of tissue samples within different embedding materials.
Knowledge and skills you might demonstrate
- Principles of specimen dissection and block selection.
- Tissue processing and embedding techniques.
- Decalcification.
- Microtomy, cryotomy.
- Macrophotography.
- Normal cellular morphology and ultrastructure of specified tissues and organ systems, including skin, building on basic anatomy and physiology.
- Basic principles of tissue preparation techniques, including factors affecting selection and their application.
- Factors that influence the quality and integrity of prepared specimens.
- The principles of specimen dissection and manipulation to expose features of interest.
- Basic principles of demonstration techniques and their rationale and hazards.
|
3 |
2 |
Apply the haematoxylin and eosin staining technique to a variety of tissue samples. |
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Apply the haematoxylin and eosin staining technique to a variety of tissue samples.
Knowledge and skills you might demonstrate
- Haematoxylin and eosin staining techniques and their application.
|
4 |
2 |
Select the appropriate tinctorial and/or histochemical staining techniques required to demonstrate a specific disease process using appropriate control material. |
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Select the appropriate tinctorial and/or histochemical staining techniques required to demonstrate a specific disease process using appropriate control material.
Knowledge and skills you might demonstrate
- Special stains to identify individual tissue/cellular components, e.g. connective tissues, nucleic acids, mucins, lipids, pigments.
- Histochemical techniques and their application.
- Potential artefacts, their identification and importance.
|
5 |
2 |
Select the appropriate impregnation techniques required to demonstrate a specific disease process using appropriate control |
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Select the appropriate impregnation techniques required to demonstrate a specific disease process using appropriate control
Knowledge and skills you might demonstrate
- The range of tinctorial and histochemical staining techniques, their selection and application to disease processes.
- The range of control materials and their appropriate use.
- Capabilities and limitations of methods, techniques and equipment.
- Principles and applications of techniques using different instrumentation.
- Correct use and application of reagents for analysis.
|
6 |
3 |
Set up and use light microscopy at various magnifications in the investigation of a range of tissue specimens. |
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Set up and use light microscopy at various magnifications in the investigation of a range of tissue specimens.
Knowledge and skills you might demonstrate
- The function of microscopic components and how to set up a microscope for investigation of histopathology specimens.
|
7 |
4 |
Select the appropriate enzyme histochemical techniques required to demonstrate a specific disease process using appropriate control material. |
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Select the appropriate enzyme histochemical techniques required to demonstrate a specific disease process using appropriate control material.
Knowledge and skills you might demonstrate
- Histochemical techniques and their application.
|
8 |
4 |
Select the appropriate immunohistochemical and/or immunofluorescence techniques and antibodies to demonstrate a specific disease process using appropriate control material. |
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Select the appropriate immunohistochemical and/or immunofluorescence techniques and antibodies to demonstrate a specific disease process using appropriate control material.
Knowledge and skills you might demonstrate
- The principles and common application of immunohistochemistry and immunofluorescence.
|
9 |
4 |
Select the appropriate molecular techniques, markers and reagents required to demonstrate a specific disease process using appropriate control material. |
View
Select the appropriate molecular techniques, markers and reagents required to demonstrate a specific disease process using appropriate control material.
Knowledge and skills you might demonstrate
- The principles and common application of molecular techniques in Histopathology.
|
10 |
1,2,3,4 |
Produce a basic interpretative report on histopathology investigations. |
View
Produce a basic interpretative report on histopathology investigations.
Knowledge and skills you might demonstrate
- The components required for a histopathology report and their relevance to patient care.
- The information to be included in an interpretative report.
- How to construct an interpretative report and the format required for presentation.
- Limits of responsibility in the authorisation and issue of interpretative reports.
- Clinical conditions that may require urgent action and how to instigate such action.
- Normal and abnormal results and their significance to the clinical question or condition.
- Relevance and importance of specificity, sensitivity, accuracy, precision and linearity in the evaluation of analytical methods.
- The range of further investigations that may be required, their purpose, capabilities and limitations.
|
11 |
1,2,5 |
Control infection risks in accordance with departmental protocols. |
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Control infection risks in accordance with departmental protocols.
Knowledge and skills you might demonstrate
- Protocols and requirements for hygiene and infection control related to the relevant range of investigations, including preparation, conduct and completion of investigation.
- Protocol for hand washing and how effective hand washing contributes to control of infection.
|
12 |
1,2,5 |
Minimise risks and hazards in compliance with health and safety policies. |
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Minimise risks and hazards in compliance with health and safety policies.
Knowledge and skills you might demonstrate
- The relevant health and safety regulations for laboratory and clinical investigations.
- The specific health and safety regulations for the specialism, type of specimen/sample and investigation.
- The potential hazards and risks and the actions to be taken to minimise these.
- Responsibilities and scope of practice of laboratory personnel involved in performing investigations and reporting those investigations to users.
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