Micromanipulation and Cryopreservation (SLS331)

10 credits

Aim of this module

This module will provide the trainee with the knowledge and understanding of the principles and practice of micromanipulation and cryopreservation and associated regulatory requirements. They will gain experience of the micromanipulation and cryopreservation of gametes and embryos in a clinical setting.

  1. Perform micromanipulation techniques.
  2. Perform cryopreservation and thawing/warming of gametes and embryos.
Number Work-based learning outcome Title Knowledge
1 1

Label and perform witnessing prior to micromanipulation of oocytes.

2 1

Use correctly the micromanipulation equipment.

3 1

Perform ICSI.

4 2

Label and perform witnessing prior to cryopreservation and following thawing/warming of semen, oocytes and embryos.

5 2

Perform sperm cryopreservation.

6 2

Thaw semen samples and compare pre-freeze and post-thaw parameters.

7 2

Perform embryo freezing and embryo vitrification.

8 2

Thaw cryopreserved samples and warm vitrified samples.

9 2

Release and receive cryopreserved gametes and embryos to other centres.

10 2

Discuss embryo development with patients and the multidisciplinary team with specific reference to the suitability for cryopreservation and the survival.

You must complete
2 Case-based discussion(s)
2 of the following DOPS / OCEs
Assessment Title Type
Set up micromanipulation equipment DOPS
Perform oocyte cryopreservation DOPS
Perform sperm cryopreservation DOPS
Perform embryo cryopreservation DOPS
Perform thawing of sperm DOPS
Thawing rewarming of cryopreserved oocytes DOPS
Thawing rewarming of cryopreserved embryos DOPS
Discuss with a patient a test thaw from a frozen semen sample and its implications for future treatment options OCE
Inform the patient of the number of embryos that are suitable for cryopreservation OCE
Discuss embryo rewarming thawing results with a patient OCE

Important information

The academic parts of this module will be detailed and communicated to you by your university. Please contact them if you have questions regarding this module and its assessments. The module titles in your MSc may not be exactly identical to the work-based modules shown in the e-portfolio. Your modules will be aligned, however, to ensure that your academic and work-based learning are complimentary.

Learning Outcomes

  1. Describe the principles and practices of micromanipulation.
  2. Explain cryobiology and describe the practices of cryopreservation and thawing.
  3. Discuss and justify the statutory and regulatory requirements of micromanipulation and cryopreservation and professional codes of practice.

Indicative Content

  • Micromanipulation equipment and methodology
  • Principles and practices of intra-cytoplasmic sperm injection (ICSI)
  • Risks and regulation associated with ICSI
  • Cryobiology, including the physical and chemical processes occurring during cryopreservation and thawing
  • Properties of cryoprotectants
  • Handling of liquid nitrogen and the appropriate health and safety regulations
  • Thermodynamics of slow freezing and vitrification
  • Physiological changes that occur in gametes and embryos during cryopreservation
  • Regulatory issues with regard to cryostorage